A-Detailed-Hierarchical-Study-of-Giardia-lamblia's-Ventral-Disc-Reveals-Novel-Microtubule-pone.0043783.s004

Description:

Whole-cell reconstruction of an attached Giardia intestinalis trophozoite using 3View®. Each slice was obtained using a microtome inside a scanning electron microscope. After each section was removed, a backscatter-signal scanning electron micrograph was recorded [18] . IMOD [56] was used to model important features of the cytoskeleton and attachment sites. Each slice is 70 nm. The majority of organelles are visible with this method: Plasma membrane (grey), median body (orange), nuclei (brown), ventral disc (green), anterior flagella (purple), caudal flagella (cyan), posterior-lateral flagella (blue), and ventral flagella (magenta). Near the ventral portion of the cell, important components of attachment are seen (bare area, lateral crest, lateral shield). Scale bar, 2 µm.