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Figure 3; Cross-sections of spermatozoa of P. shantungensis. A Acrosome, showing the dothideoid acrosome B–E serial cross-sections of head showing the dothideoid acrosome (a), the nucleus (N), and the plasma membrane (pm) F oval nucleus (N) G–N nucleus-flagellum transition region, showing the nucleus (N), mitochondrial derivatives (md), accessory bodies (ab), axoneme (ax). The asterisk indicates the centriolar adjunct (ca). Scale bars: 0.5 µm (B–D); 0.2 µm (A, K, L, N); 0.1 µm (E–J, M).
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Figure 4; Cross-sections of the sperm flagellum of P. shantungensis. A–D Flagella, showing axoneme (ax), fishhook-shaped accessory bodies (ab), D-shaped mitochondrial derivatives (md), containing oval lucent region (1), serrated electron-dense region (2) and mitochondrial cristae region (3) E–F flagellum, mitochondrial derivatives slowly disappear, axonemes (ax) become disordered, accessory bodies (ab) become smaller G axoneme, showing the typical 9 + 9 + 2 pattern, nine outermost accessory microtubules (am), nine doublet microtubules (dm) and two innermost central microtubules (cm) H Showing doublet microtubules finally disappearing. Scale bars: 2 µm (A); 0.5 µm (B); 0.1 µm (C–H).
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Figure 2; Longitudinal sections of spermatozoa of P. shantungensis. A, B Spermatozoa, showing acrosome (a), nucleus (N), arrow indicates connection area between acrosome and nucleus C nucleus-flagellum transition, showing nucleus (N), mitochondrial derivatives (md), accessory body (ab), axoneme (ax), arrow indicates centriole, triangular arrowhead indicates centriolar adjunct D, E flagella of sperm, showing axoneme (ax), mitochondrial derivatives (md) and cristae (arrow). Scale bars: 2 µm (A); 0.5 µm (B, D); 0.1 µm (C, E).
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Figure 1; TEM and light micrographs of spermatozoa and spermatodesms of P. shantungensis. A, B Light micrographs of spermatodesm and spermatozoa C light micrograph of a single spermatozoon with the head (H, arrow) and wavy flagellum (F) D, E TEM micrographs of cross-sections of spermatozoa, showing the acrosome (a) and nucleus (N). Arrow shows head cluster, asterisk indicates the acrosome. Scale bars: 20 µm (A–C); 2 µm (D); 0.5µm (E).
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