Summary[edit] Description: English: Queller/Strassmann Lab - Photographed by Usman Bashir. Date: 12 November 2015, 15:15:25. Source: Own work. Author: Usman Bashir. Washington University in St. Louis Licensing[edit] : This file is licensed under the Creative CommonsAttribution-Share Alike 4.0 International license.:. https://creativecommons.org/licenses/by-sa/4.0 CC BY-SA 4.0 Creative Commons Attribution-Share Alike 4.0 truetrue.
Summary[edit] Description: English: Kuzdzal-Fick et al. experimentally evolved replicate lines of D. discoideum under treatments enforcing low relatedness. Transfers were performed using 106 spores gathered from across the plate, effectively mixing the population each transfer. Cheater mutants were repeatedly exposed to new partners to exploit and so prospered. Obligate cheaters incapable of fruiting on their own readily evolved. Eventually, the pressure exerted by cheaters caused other genotypes to evolve ‘noble resistance’ – these strains could resist cheating without being cheaters themselves. Date: 31 May 2019. Source: Own work. Author: Tyler Larsen.
Summary[edit] Description: English: Queller/Strassmann Lab - Photographed by Usman Bashir. Date: 12 November 2015, 12:34:04. Source: Own work. Author: Usman Bashir. Washington University in St. Louis Licensing[edit] : This file is licensed under the Creative CommonsAttribution-Share Alike 4.0 International license.:. https://creativecommons.org/licenses/by-sa/4.0 CC BY-SA 4.0 Creative Commons Attribution-Share Alike 4.0 truetrue.
Summary[edit] Description: English: Chimeras readily form in laboratory populations of D. discoideum. While forming a chimera may sometimes be beneficial if it results in a larger aggregate, chimeric slugs are less motile than clonal slugs of the same size, which may result from conflict between genotypes within the slug. Chimeric fruiting bodies also are subjected to the risk of being cheated upon, and may produce smaller stalks than clonal fruiting bodies. Date: 31 May 2019. Source: Own work. Author: Tyler Larsen.
Summary[edit] Description: English: Structured growth of an initially well-mixed (low-relatedness) population can produce patches of high relatedness due to the space constraints imposed on densely-growing cells. Cells on the periphery expand outward into radial sectors of clonal daughter cells. Whether genetic demixing can occur in natural soil is unknown. Date: 31 May 2019. Source: Own work. Author: Tyler Larsen.
Summary[edit] Description: English: Queller/Strassmann Lab - Photographed by Usman Bashir. Date: 12 November 2015, 15:27:35. Source: Own work. Author: Usman Bashir. Washington University in St. Louis Licensing[edit] : This file is licensed under the Creative CommonsAttribution-Share Alike 4.0 International license.:. https://creativecommons.org/licenses/by-sa/4.0 CC BY-SA 4.0 Creative Commons Attribution-Share Alike 4.0 truetrue.
Hoeller O, Toettcher J, Cai H, Sun Y, Huang C, Freyre M, Zhao M, Devreotes P, Weiner O
Wikimedia Commons
Onset of oscillations after rapamycin-induced Gβ sequestration. Most behaviors described in this paper are apparent in this movie of the Gβ sequestration strain expressing the F-actin binding probe LimE-GFP. The movie is split (LimE-GFP on the left; FRB-RFP-Gβ on the right). After frame 300, addition of 1 μM rapamycin (labeled) induces sequestration of Gβ. This starts to show as visible clustering of Gβ about halfway through the movie. Within 40 s, LimE-GFP begins rapid whole-field oscillations that alternate with polarized accumulation of F-actin. Toward the end of the movie, circular rotations of LimE-GFP can be observed. The movie was recorded at 1 frame/second and is played back at 30 frames/second. Portions of this movie correspond to S9 Fig.
Summary[edit] Description: English: Queller/Strassmann Lab - Photographed by Usman Bashir. Date: 12 November 2015, 16:28:53. Source: Own work. Author: Usman Bashir. Washington University in St. Louis Licensing[edit] : This file is licensed under the Creative CommonsAttribution-Share Alike 4.0 International license.:. https://creativecommons.org/licenses/by-sa/4.0 CC BY-SA 4.0 Creative Commons Attribution-Share Alike 4.0 truetrue.
Summary[edit] Description: English: A) In the absence of cheating, two genotypes that aggregate together in a 50:50 ratio will each contribute half of the resulting fruiting body’s stalk and half of its sorus. B) Fixed cheaters produce a higher spore:stalk ratio when grown clonally or in a chimera. These strategies may or may not have evolved due to the social benefits of cheating in chimeras. C) Facultative cheaters take advantage of other genotypes in a chimera by either forcing other genotypes to reduce their spore:stalk ratio (coercion) or increasing their own spore:stalk ratio (self-promotion). D) Obligate cheaters depend on the presence of other genotypes to fruit. They have been observed in experimentally evolved populations in the laboratory but do not appear to survive in nature. Date: 31 May 2019. Source: Own work. Author: Tyler Larsen.
Summary[edit] Description: English: Queller/Strassmann Lab - Photographed by Usman Bashir. Date: 12 November 2015, 12:38:13. Source: Own work. Author: Usman Bashir. Washington University in St. Louis Licensing[edit] : This file is licensed under the Creative CommonsAttribution-Share Alike 4.0 International license.:. https://creativecommons.org/licenses/by-sa/4.0 CC BY-SA 4.0 Creative Commons Attribution-Share Alike 4.0 truetrue.
Delivery of the V-ATPase to a new phagosome. The cell is expressing VatM-GFP and has been incubated with TRITC-dextran to label endosomes. The uptake of a heat-killed yeast is shown.
Summary[edit] Description: English: Dictyostelium discoideum fruiting bodies at various stages of development. False-color fluorescence image, focus stack composite. Photo: jeff smith. Date: 16 February 2016. Source: Own work. Author: jeff smith.
Labeling of a macropinosome with GFP-2FYVE, a biosensor for PI(3)P. The cell is expressing GFP-2FYVE and mRFP-LimEΔ. The nascent macropinosome is initially labeled with the actin marker. Actin disappears and 2FYVE-GFP binding begins after about one minute. It persists for another 2 or 3 minutes as the macropinosome elongates and fragments, behavior consistent with the early or sorting endosome stage.
Summary[edit] Description: English: Queller/Strassmann Lab - Photographed by Usman Bashir. Date: 12 November 2015, 16:35:55. Source: Own work. Author: Usman Bashir. Washington University in St. Louis Licensing[edit] : This file is licensed under the Creative CommonsAttribution-Share Alike 4.0 International license.:. https://creativecommons.org/licenses/by-sa/4.0 CC BY-SA 4.0 Creative Commons Attribution-Share Alike 4.0 truetrue.
Hoeller O, Toettcher J, Cai H, Sun Y, Huang C, Freyre M, Zhao M, Devreotes P, Weiner O
Wikimedia Commons
Kymograph (t-stack) of actin accumulation in a Gβ-sequestered cell (+ rapamycin). 360-degree rotation of a t-stack showing F actin accumulation at the cortex (visualized with LimE-GFP) during polar and apolar phases. Movie corresponds to Fig 7B and is played at 72 frames/second. Vertical axis represents time (first time point on top, last time point on bottom).
Summary[edit] Description: English: Queller/Strassmann Lab - Photographed by Usman Bashir. Date: 12 November 2015, 16:34:06. Source: Own work. Author: Usman Bashir. Washington University in St. Louis Licensing[edit] : This file is licensed under the Creative CommonsAttribution-Share Alike 4.0 International license.:. https://creativecommons.org/licenses/by-sa/4.0 CC BY-SA 4.0 Creative Commons Attribution-Share Alike 4.0 truetrue.
Summary[edit] Description: English: Queller/Strassmann Lab - Photographed by Usman Bashir. Date: 12 November 2015, 17:44:19. Source: Own work. Author: Usman Bashir. Washington University in St. Louis Licensing[edit] : This file is licensed under the Creative CommonsAttribution-Share Alike 4.0 International license.:. https://creativecommons.org/licenses/by-sa/4.0 CC BY-SA 4.0 Creative Commons Attribution-Share Alike 4.0 truetrue.
Acquisition of GFP-2FYVE label by a vacuole that has separated from a phagosome just prior to premature exocytosis. The cell is expressing GFP-2FYVE and mRFP-LimEΔ; the phagosome contains a FITC-yeast.
Summary[edit] Description: English: Queller/Strassmann Lab - Photographed by Usman Bashir. Date: 30 October 2015, 14:55:29. Source: Own work. Author: Usman Bashir. Washington University in St. Louis Licensing[edit] : This file is licensed under the Creative CommonsAttribution-Share Alike 4.0 International license.:. https://creativecommons.org/licenses/by-sa/4.0 CC BY-SA 4.0 Creative Commons Attribution-Share Alike 4.0 truetrue.
