Whole-cell reconstruction model showing relationships between cytoskeletal elements. The Video starts with the raw data, then transitions into the modeled data created by IMOD [56] . The ventral portion of the cell contains most of the cytoskeletal elements—the ventral disc (VD), median body (MB), and 4 pairs of flagella (anterior flagella, AFL; caudal flagella, CFL; posterior-lateral flagella, PFL; ventral flagella, VFL) as well as the two nuclei (N). The bulk of the cytoskeletal elements are at the ventral portion of the cell—the attachment site to the host microvili. Scale bar, 2 µm.
Tomographic reconstruction of 3 serial-montaged sections of a Giardia trophozoite. Each slice is 3 nm in the Z-plane. The transition zone between sections looks like a jump. Anterior flagella, AFL (purple); caudal flagella, CFL (cyan); posterior-lateral flagella, PFL (blue); ventral disc microtubules, MT (white); microribbons, MR (green); dense bands, DB; supernumerary microtubules, SMT (yellow). Margin-facing and axis-facing sides are shown for orientation. This volume is about ∼11 µm 3 of the entire disc, which has a volume of ∼54 µm 3 . Scale bar, 500 nm.
Whole-cell reconstruction of an attached Giardia intestinalis trophozoite using 3View®. Each slice was obtained using a microtome inside a scanning electron microscope. After each section was removed, a backscatter-signal scanning electron micrograph was recorded [18] . IMOD [56] was used to model important features of the cytoskeleton and attachment sites. Each slice is 70 nm. The majority of organelles are visible with this method: Plasma membrane (grey), median body (orange), nuclei (brown), ventral disc (green), anterior flagella (purple), caudal flagella (cyan), posterior-lateral flagella (blue), and ventral flagella (magenta). Near the ventral portion of the cell, important components of attachment are seen (bare area, lateral crest, lateral shield). Scale bar, 2 µm.
Summary[edit] Description: English: Video of co-culture at day 0. The co-culture consisting of Caco-2 cells and IC-21 macrophages was assembled as described and incubated for 3 days. Giardia trophozoites were then added at 100,000 total parasites and a video was taken immediately. Date: 2013. Source: Video S1 from Fisher B, Estrano C, Cole J (2013). "Modeling Long-Term Host Cell-Giardia lamblia Interactions in an In Vitro Co-Culture System". PLOS ONE. DOI:10.1371/journal.pone.0081104. PMID24312526. PMC: 3849038. Author: Fisher B, Estrano C, Cole J. Permission (Reusing this file): : This file is licensed under the Creative CommonsAttribution 3.0 Unported license.:. You are free: to share – to copy, distribute and transmit the work to remix – to adapt the work Under the following conditions: attribution – You must attribute the work in the manner specified by the author or licensor (but not in any way that suggests that they endorse you or your use of the work). http://creativecommons.org/licenses/by/3.0 CC BY 3.0 Creative Commons Attribution 3.0 truetrue. Provenance: This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.: .